Tiny Toon Adventures was a cartoon set in the fictional city of Acme Acres, where most of the Tiny Toons and Looney Tunes characters live. The characters attended Acme Looniversity, a school whose faculty primarily consists of the mainstays of the classic Warner Bros. cartoons, such as Bugs Bunny, Daffy Duck, Sylvester the Cat and Elmer Fudd. In the series, the university was founded to teach cartoon characters how to become funny. The school is not featured in every episode, as not all of its storylines are school-centric.
Like the Looney Tunes, the series was derived from cartoon violence (e.g. anvils falling on someone, liberal use of explosives) and slapstick. The series parodied and referenced the current events of the early 1990s and Hollywood culture. Occasionally, episodes would delve into veiled ethical and morality stories of ecology, self-esteem, and crime.